Anticorps Polyclonal de lapin anti-ATP1A1-Specific

• Phare
• Validé par KD/KO

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 55187-1-AP

Synonymes

ATP1A1, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HEK-293 using 55187-1-AP

  WB result of ATP1A1-Specific antibody (55187-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1-Specific transfected HEK-293 cells.

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  WB analysis of mouse brain using 55187-1-AP

  37°C incubated mouse brain membrane extract lysate and 37°C incubated mouse heart membrane extract lysate were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 55187-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55187-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55187-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55187-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 55187-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1-Specific antibody (55187-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Caco-2 using 55187-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 55187-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1-Specific Polyclonal antibody (55187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris incubé à 37 °C, cellules HEK-293, cellules HepG2, cellules MCF-7, cellules Neuro-2a, tissu cardiaque de souris incubé à 37 °C
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer du foie humain, tissu de côlon humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules Caco-2
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IF: See 7 publications below

Informations sur le produit

55187-1-AP cible ATP1A1-Specific dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: NM_000701
• Symbole du gène: ATP1A1
• Identification du gène (NCBI): 476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Publications

Species	Application	Title
human	IF	Autophagy; Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury; Authors - Yanping Li
		Nat Commun; CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.; Authors - Tiantian Tang
human	WB	Adv Healthc Mater; Effect of Nanoparticle Rigidity on the Interaction of Stromal Membrane Particles with Leukemia Cells; Authors - Sander de Weerd
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
mouse	IF	Mol Biol Cell; Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.; Authors - Karolina Plössl
human	IF	Sci Rep; Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.; Authors - Yan Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/Antibodies dilution solution: 0.05% saponin, 5% horse serum in PBS. ATP1A1 antibody was diluted 1:100 in saponin solution and incubated on cells at room temperature for 1 hour, followed by 1 hour incubation with anti-rabbit conjugated to AlexaFluor 488 (1:200).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

ATP1A1-Specific Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:55187-1-AP)

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