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ATP1A1 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ATP1A1 Antibody WB mouse heart tissue 14418-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATP1A1 Antibody IF Caco-2 cells 14418-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed Caco-2 cells using 14418-1-AP (ATP1A1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ATP1A1 Antibody IHC human liver cancer tissue 14418-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

ATP1A1 Antibody IHC human liver cancer tissue 14418-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

ATP1A1 Antibody IP HEK-293 cells 14418-1-AP

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
mouse heart tissue, human colon tissue, A549 cells, COLO 320 cells
Positive IP detected in:
HEK-293 cells, A431 cells
Positive IHC detected in:
human liver cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Caco-2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATP1A1 fusion protein Ag5763
Full name:
ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight:
113 kDa
Observed molecular weight:
100-110 kDa
GenBank accession number:
BC050359
Gene ID (NCBI):
476
Gene symbol
ATP1A1
Synonyms
ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

Background

ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ATP1A1 Rabbit Polyclonal antibody

Gongsheng (Verified Customer), 12-18-2018
Applications
Immunofluorescence,
Primary Antibody Dilution
1:100

Cell/Tissue Type
Human epithelium
Lot Number

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