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ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14418-1-AP

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1


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  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    37°C incubated HEK-293 membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis using 14418-1-AP

    37°C incubated mouse brain total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    WB analysis of HEK-293 using 14418-1-AP

    WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IP experiment of HEK-293 using 14418-1-AP

    IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

  • Immunoprecipitation (IP) experiment of A431 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IP experiment of A431 using 14418-1-AP

    IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human liver cancer using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATP1A1 Polyclonal antibody (14418-1-AP)

    IHC staining of human ovary tumor using 14418-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IF Staining of Caco-2 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Polyclonal antibody (14418-1-AP)

    IF Staining of HEK-293 using 14418-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

"ATP1A1 Antibodies" Comparison

View side-by-side comparison of ATP1A1 antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, rat heart tissue
Positive IP detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:400
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14418-1-AP targets ATP1A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
Gene ID (NCBI) 476
RRIDAB_2227873
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for ATP1A1 antibody 14418-1-APDownload protocol
IHC protocol for ATP1A1 antibody 14418-1-APDownload protocol
IF protocol for ATP1A1 antibody 14418-1-APDownload protocol
IP protocol for ATP1A1 antibody 14418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Authors - Teng Ma
WB

Adv Sci (Weinh)

Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis

Authors - Wenqi Sun
humanWB

Theranostics

Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer

Authors - Chen Li
humanWB

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang
humanWB

Nat Commun

A broadly neutralizing humanized ACE2-targeting antibody against SARS-CoV-2 variants.

Authors - Yanyun Du
humanWB

Nat Commun

Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.

Authors - Yanyun Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aleix (Verified Customer) (04-26-2022)

We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Skin
FH

Gongsheng (Verified Customer) (12-18-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human epithelium
ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)