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ATP1A1 Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ATP1A1 Antibody WB mouse heart tissue 14418-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATP1A1 Antibody IF Caco-2 cells 14418-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed Caco-2 cells using 14418-1-AP (ATP1A1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ATP1A1 Antibody IHC human liver cancer tissue 14418-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

ATP1A1 Antibody IHC human liver cancer tissue 14418-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

ATP1A1 Antibody IP HEK-293 cells 14418-1-AP

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
mouse heart tissue, human colon tissue, A549 cells, COLO 320 cells
Positive IP detected in:
HEK-293 cells, A431 cells
Positive IHC detected in:
human liver cancer tissue, human ovary tumor tissue
Positive IF detected in:
Caco-2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATP1A1 fusion protein Ag5763
Full name:
ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight:
113 kDa
Observed molecular weight:
100-110 kDa
GenBank accession number:
BC050359
Gene ID (NCBI):
476
Gene symbol
ATP1A1
Synonyms
ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

Background

ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.


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