"ATP1A1 Antibodies" Comparison
View side-by-side comparison of ATP1A1 antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, 37°C incubated HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human cervical cancer tissue, human colon tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue, human cervical cancer tissue |
| Positive IF/ICC detected in | Caco-2 cells, HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
| Positive CoIP detected in | A431 cells |
For optimal WB detection with 14418-1-AP, we do not recommend boiling the sample after lysis.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Co-Immunoprecipitation (CoIP) | COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14418-1-AP targets ATP1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5763 Product name: Recombinant human ATP1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species |
| Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| Calculated Molecular Weight | 113 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC050359 |
| Gene Symbol | ATP1A1 |
| Gene ID (NCBI) | 476 |
| ENSEMBL Gene ID | ENSG00000163399 |
| RRID | AB_2227873 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
| IF protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
| IHC protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
| IP protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
| WB protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ATP1A1 Polyclonal antibody (Cat# 14418-1-AP) has accumulated 362 total citations. Publications appear in top-tier journals including Nature, Science, Cell, Immunity, Nature Communications, Molecular Cell, and Advanced Science, among over 80 distinct journals. Research fields span oncology, neuroscience, cardiology, immunology, metabolism, nephrology, nanomedicine, diabetes, and hepatology. The antibody is predominantly used for Western blot validation across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Science A subcellular map of translational machinery composition and regulation at the single-molecule level | ||
Cell High-resolution spatially resolved proteomics of complex tissues based on microfluidics and transfer learning |
Reviews
What customers say
Users report generally positive results with this antibody. One reviewer noted strong Western Blot signal in human cell lysate, though the target also appeared in the membrane fraction. Another found it functional for immunofluorescence on skin frozen sections but described the signal as weak. A third reviewer gave a top rating for IF on human epithelium at 1:100 dilution with no additional comments. Overall, the antibody performs well in WB and IF, with some variability in signal strength depending on application and tissue type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jimmy (Verified Customer) (02-27-2024) | Strong signal against human cell lysate, but target protein also show up in cell membrane fraction.
|
Aleix (Verified Customer) (04-26-2022) | We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.
|
Gongsheng (Verified Customer) (12-18-2018) |
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