ATP1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP1A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

53

Reviews (1)

Cat no : 14418-1-AP

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

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Validation Data Gallery

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  WB analysis using 14418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 14418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using 14418-1-AP (ATP1A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IP experiment of HEK-293 using 14418-1-AP

  IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells, A431 cells
• Positive IHC detected in: human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 47 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

14418-1-AP targets ATP1A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, swine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1A1 fusion protein Ag5763
• Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Calculated molecular weight: 113 kDa
• Observed molecular weight: 100-110 kDa
• GenBank accession number: BC050359
• Gene symbol: ATP1A1
• Gene ID (NCBI): 476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

Publications

Species	Application	Title
rat	WB	Biomed Pharmacother; CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.; Authors - Xuezhi Yang
human	WB	Aging (Albany NY); Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract.; Authors - Yu Yan
mouse	WB	Cell Death Dis; CXCR7 regulates epileptic seizures by controlling the synaptic activity of hippocampal granule cells.; Authors - Tao Xu
mouse	WB	Cells; GRK2 Mediated Abnormal Transduction of PGE2-EP4-cAMP-CREB Signaling Induces the Imbalance of Macrophages Polarization in Collagen-Induced Arthritis Mice.; Authors - Xuezhi Yang
human	WB	Front Oncol; The Binding of PD-L1 and Akt Facilitates Glioma Cell Invasion Upon Starvation via Akt/Autophagy/F-Actin Signaling.; Authors - Ruo Qiao Chen
mouse	WB	J Diabetes Res; Identification of Protein Kinase C Isoforms Involved in Type 1 Diabetic Encephalopathy in Mice.; Authors - Jiayin Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gongsheng (Verified Customer) (12-18-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human epithelium

ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)

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