|Positive WB detected in||mouse heart tissue, HEK-293 cells|
|Positive IP detected in||HEK-293 cells, A431 cells|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14418-1-AP targets ATP1A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP1A1 fusion protein Ag5763|
|Full Name||ATPase, Na+/K+ transporting, alpha 1 polypeptide|
|Calculated molecular weight||113 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC050359|
|Gene ID (NCBI)||476|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.
|Product Specific Protocols|
|WB protocol for ATP1A1 antibody 14418-1-AP||Download protocol|
|IHC protocol for ATP1A1 antibody 14418-1-AP||Download protocol|
|IF protocol for ATP1A1 antibody 14418-1-AP||Download protocol|
|IP protocol for ATP1A1 antibody 14418-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Gongsheng (Verified Customer) (12-18-2018)