- Featured Product
- KD/KO Validated
ATP1A1 Polyclonal antibody
ATP1A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14418-1-AP
"ATP1A1 Antibodies" Comparison
View side-by-side comparison of ATP1A1 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in
|mouse heart tissue, HEK-293 cells, 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, rat heart tissue
|Positive IP detected in
|HEK-293 cells, A431 cells
|Positive IHC detected in
|human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Caco-2 cells, HEK-293 cells
|Western Blot (WB)
|WB : 1:5000-1:20000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:400
|IF : 1:400-1:1600
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14418-1-AP targets ATP1A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, chicken, pig
|Host / Isotype
|Rabbit / IgG
|ATP1A1 fusion protein Ag5763
|ATPase, Na+/K+ transporting, alpha 1 polypeptide
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.
|Product Specific Protocols
|WB protocol for ATP1A1 antibody 14418-1-AP
|IHC protocol for ATP1A1 antibody 14418-1-AP
|IF protocol for ATP1A1 antibody 14418-1-AP
|IP protocol for ATP1A1 antibody 14418-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Aleix (Verified Customer) (04-26-2022)
We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.
Gongsheng (Verified Customer) (12-18-2018)