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- KD/KO Validated
ATP1A1 Polyclonal antibody
ATP1A1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 14418-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, HEK-293 cells |
Positive IP detected in | HEK-293 cells, A431 cells |
Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Caco-2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 54 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
Product Information
14418-1-AP targets ATP1A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ATP1A1 fusion protein Ag5763 |
Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
Calculated molecular weight | 113 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC050359 |
Gene symbol | ATP1A1 |
Gene ID (NCBI) | 476 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
IHC protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
IF protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
IP protocol for ATP1A1 antibody 14418-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation. | ||
Aging (Albany NY) Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract. | ||
Cell Death Dis CXCR7 regulates epileptic seizures by controlling the synaptic activity of hippocampal granule cells. | ||
Cells GRK2 Mediated Abnormal Transduction of PGE2-EP4-cAMP-CREB Signaling Induces the Imbalance of Macrophages Polarization in Collagen-Induced Arthritis Mice. | ||
Front Oncol The Binding of PD-L1 and Akt Facilitates Glioma Cell Invasion Upon Starvation via Akt/Autophagy/F-Actin Signaling. | ||
J Diabetes Res Identification of Protein Kinase C Isoforms Involved in Type 1 Diabetic Encephalopathy in Mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Gongsheng (Verified Customer) (12-18-2018) |
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