ATP1A1 Antibody 14418-1-AP

Tested Applications

Applications:

ELISA, IHC, IP, WB
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mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP( ATP1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse heart tissue, A549 cells, human colon tissue, COLO 320 cells

Positive IP detected in:

HEK-293 cells, A431 cells

Positive IHC detected in:

human liver cancer tissue, human liver cancer tissue, human ovary tumor tissue

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IHC : 1:20-1:400

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ATP1A1 fusion protein Ag5763

Full name:

ATPase, Na+/K+ transporting, alpha 1 polypeptide

Calculated molecular weight:

113 kDa

Observed molecular weight:

100-110 kDa

GenBank accession number:

BC050359

Gene ID (NCBI):

476

Gene symbol

ATP1A1

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

Background

ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

Showing 1- [6] of [9] Publications

Author:		Lai Guangrui G
Pubmed ID:		22918876
Journal:		Mol Endocrinol
Title:		20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.
Application:		WB

Species:		mouse

Author:		Qinmei Xiong
Pubmed ID:		25616976
Journal:		J Am Heart Assoc
Title:		Arrhythmogenic Cardiomyopathy in a Patient With a Rare Loss-of-Function KCNQ1 Mutation.
Application:		WB

Species:		human

Author:		Xiaofei Yan
Pubmed ID:		27563007
Journal:		Acta Biochim Biophys Sin (Shanghai)
Title:		Activation of Na+/K+-ATPase attenuates high glucose-induced H9c2 cell apoptosis via suppressing ROS accumulation and MAPKs activities by DRm217.
Application:		WB

Species:		rat

Author:		Prasenjit Manna
Pubmed ID:		28063949
Journal:		Arch Biochem Biophys
Title:		Vitamin D supplementation inhibits oxidative stress and upregulate SIRT1/AMPK/GLUT4 cascade in high glucose-treated 3T3L1 adipocytes and in adipose tissue of high fat diet-fed diabetic mice.
Application:		WB

Species:		MOUSE

Author:		Xiaofei Yan
Pubmed ID:		28181111
Journal:		Apoptosis
Title:		Activation of Na(+)-K(+)-ATPase with DRm217 attenuates oxidative stress-induced myocardial cell injury via closing Na(+)-K(+)-ATPase/Src/Ros amplifier.
Application:		WB

Species:		rat

Author:		Yuetao Wen
Pubmed ID:		28299629
Journal:		Neurochem Res
Title:		Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.
Application:		WB

Species:		human

WB Figures

A549 cells were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

COLO 320 cells were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

human colon tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP( ATP1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

IP Figures

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

ATP1A1 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [9] Publications

WB Figures

IHC Figures

IP Figures

ATP1A1 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Tested Applications

Product Information

Immunogen Information

Background