Name: ATP1A1 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

ATP1A1 Antibody WB mouse heart tissue 14418-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATP1A1 Antibody WB HEK-293 cells 14418-1-AP

WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells

ATP1A1 Antibody IF Caco-2 cells 14418-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed Caco-2 cells using 14418-1-AP (ATP1A1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ATP1A1 Antibody IHC human liver cancer tissue 14418-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

ATP1A1 Antibody IP HEK-293 cells 14418-1-AP

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

Species specificity:

human, mouse, rat, pig

Positive WB detected in:

mouse heart tissue, human colon tissue, A549 cells, COLO 320 cells, HEK-293 cells

Positive IP detected in:

HEK-293 cells, A431 cells

Positive IHC detected in:

human liver cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

Caco-2 cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IHC : 1:20-1:400

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ATP1A1 fusion protein Ag5763

Full name:

ATPase, Na+/K+ transporting, alpha 1 polypeptide

Calculated molecular weight:

113 kDa

Observed molecular weight:

100-110 kDa

GenBank accession number:

BC050359

Gene ID (NCBI):

476

Gene symbol

ATP1A1

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

Background

ATP1A1 is the catalytic component of Na+/K+-ATPase which is is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ATP1A1 Rabbit Polyclonal antibody

Gongsheng (Verified Customer), 12-18-2018

Applications

Immunofluorescence,

Primary Antibody Dilution

1:100

ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)

Cell/Tissue Type

Human epithelium

Lot Number

Showing 1- [6] of [23] Publications

Author:		Lai Guangrui G
Pubmed ID:		22918876
Journal:		Mol Endocrinol
Title:		20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.
Application:		WB

Species:		mouse

Author:		Qinmei Xiong
Pubmed ID:		25616976
Journal:		J Am Heart Assoc
Title:		Arrhythmogenic Cardiomyopathy in a Patient With a Rare Loss-of-Function KCNQ1 Mutation.
Application:		WB

Species:		human

Author:		Xiaofei Yan
Pubmed ID:		27563007
Journal:		Acta Biochim Biophys Sin (Shanghai)
Title:		Activation of Na+/K+-ATPase attenuates high glucose-induced H9c2 cell apoptosis via suppressing ROS accumulation and MAPKs activities by DRm217.
Application:		WB

Species:		rat

Author:		Prasenjit Manna
Pubmed ID:		28063949
Journal:		Arch Biochem Biophys
Title:		Vitamin D supplementation inhibits oxidative stress and upregulate SIRT1/AMPK/GLUT4 cascade in high glucose-treated 3T3L1 adipocytes and in adipose tissue of high fat diet-fed diabetic mice.
Application:		WB

Species:		MOUSE

Author:		Xiaofei Yan
Pubmed ID:		28181111
Journal:		Apoptosis
Title:		Activation of Na(+)-K(+)-ATPase with DRm217 attenuates oxidative stress-induced myocardial cell injury via closing Na(+)-K(+)-ATPase/Src/Ros amplifier.
Application:		WB

Species:		rat

Author:		Yuetao Wen
Pubmed ID:		28299629
Journal:		Neurochem Res
Title:		Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.
Application:		WB

Species:		human

WB Figures

ATP1A1 Antibody WB A549 cells 14418-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

ATP1A1 Antibody WB COLO 320 cells 14418-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells

ATP1A1 Antibody WB human colon tissue 14418-1-AP

human colon tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP(ATP1A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP( ATP1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

ATP1A1 Antibody IHC human ovary tumor tissue 14418-1-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens)

IP Figures

ATP1A1 Antibody IP A431 cells 14418-1-AP

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.

IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

IF Figures

Immunofluorescent analysis of ( -20°C Ethanol) fixed Caco-2 cells using 14418-1-AP (ATP1A1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ATP1A1 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

ATP1A1 Rabbit Polyclonal antibody

Showing 1- [6] of [23] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

ATP1A1 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 14418-1-AP

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Related Primary Antibodies

Special offer: Add control/tag antibody for $99

Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

ATP1A1 Rabbit Polyclonal antibody