Anticorps Monoclonal anti-c-Kit/CD117
c-Kit/CD117 Monoclonal Antibody for ELISA
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Humain
Applications
ELISA
Conjugaison
Non conjugué
CloneNo.
2E8G4
N° de cat : 60169-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Dilution recommandée
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
60169-1-Ig cible c-Kit/CD117 dans les applications de ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Mouse / IgG2b |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Masse moléculaire calculée | 110 kDa |
| Poids moléculaire observé | 120 kDa |
| Numéro d’acquisition GenBank | BC071593 |
| Symbole du gène | KIT |
| Identification du gène (NCBI) | 3815 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.
