Anticorps Monoclonal anti-Beta Tubulin
Beta Tubulin Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, namatode, poisson-zèbre, porc, rat, souris et plus (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugaison
Non conjugué
310
CloneNo.
1D4A4
N° de cat : 66240-1-Ig
Synonymes
"Beta Tubulin Antibodies" Comparison
View side-by-side comparison of Beta Tubulin antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HT-1080, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NCCIT, cellules NIH/3T3, cellules ROS1728 |
| Résultats positifs en IP | cellules HeLa |
| Résultats positifs en IHC | tissu cérébral humain, tissu cérébral de rat, tissu de cancer du sein humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | cellules HeLa, cellules HepG2 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:20-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 282 publications below |
| IHC | See 1 publications below |
| IF | See 19 publications below |
| IP | See 3 publications below |
Informations sur le produit
66240-1-Ig cible Beta Tubulin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, namatode, poisson-zèbre, porc, rat, souris
| Réactivité | Humain, namatode, poisson-zèbre, porc, rat, souris |
| Réactivité citée | rat, Humain, Lapin, poisson-zèbre, porc, poulet, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Beta Tubulin Protéine recombinante Ag0117 |
| Nom complet | tubulin, beta 3 |
| Masse moléculaire calculée | 450 aa, 50 kDa |
| Poids moléculaire observé | 50-55 kDa |
| Numéro d’acquisition GenBank | BC000748 |
| Symbole du gène | TUBB3 |
| Identification du gène (NCBI) | 10381 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.
What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?
The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).
How to use beta-tubulin as a loading control
Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.
What drugs can influence beta-tubulin and organization of microtubules?
Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.
Is beta-tubulin post-translationally modified?
Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IHC protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IF protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| IP protocol for Beta Tubulin antibody 66240-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Science A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements. | ||
Adv Mater A Physiologically Responsive Nanocomposite Hydrogel for Treatment of Head and Neck Squamous Cell Carcinoma via Proteolysis-targeting Chimeras Enhanced Immunotherapy | ||
Cell Metab Mapping the single-cell transcriptomic response of murine diabetic kidney disease to therapies. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andrea (Verified Customer) (01-11-2023) | Antibody were incubated for 2h at room temperature. Provide a specific tubulin signal
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FH Emma (Verified Customer) (11-29-2021) | Very clean antibody and runs at the right size by western blot.
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FH Raul (Verified Customer) (08-20-2021) | Very good antibody producing a single strong band at ~55kDa
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FH Yu Siong (Verified Customer) (08-18-2020) | Western blot of 4 bands: 4 different treatments.
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FH Kathryn (Verified Customer) (12-10-2019) | Used 1:20000 in 5% BSA on a small amount of neonatal mouse ovary. Very clean and specific, and great that it works at such a low concentration.
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FH Sara (Verified Customer) (10-09-2019) | This antibody works great in WB. We use it as loading control in whole cell lysates (mice B cell line), 1:10.000 O/N at 4°C.
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FH VLADIMIR (Verified Customer) (09-25-2019) | B-tubulin IgG2a were used to prepare Fab' conjugated with Alexa 647-maleimide
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FH Jeffery (Verified Customer) (08-01-2019) | Very clean antibody. Works exceptionally well. Runs right at the 50kd marker.
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FH Shashirekha (Verified Customer) (05-09-2019) | Works great!
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FH Zunair (Verified Customer) (12-03-2018) |
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