Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10094-1-AP

Synonyms

beta tubulin, B-tubulin, tbb5, TUBB, β-tubulin


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Applications:
  • Western Blot (WB) analysis of various lysates using Beta Tubulin Polyclonal antibody (10094-1-AP)

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (Beta Tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta Tubulin Polyclonal antibody (10094-1-AP)

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta Tubulin Polyclonal antibody (10094-1-AP)

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta Tubulin Polyclonal antibody (10094-1-AP)

    WB analysis using 10094-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IP experiment of mouse brain using 10094-1-AP

    IP Result of anti-beta Tubulin (IP:10094-1-AP, 3ug; Detection:10094-1-AP 1:1000) with mouse brain tissue lysate 7500ug.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of rat brain using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of rat brain using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of rat testis using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of rat testis using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of human cerebellum using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of human cerebellum using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of mouse brain using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of mouse brain using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of human colon using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IHC staining of human colon using 10094-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta Tubulin Polyclonal antibody (10094-1-AP)

    IF Staining of HepG2 using 10094-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Beta Tubulin Polyclonal antibody (10094-1-AP)

    FC experiment of HepG2 using 10094-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug beta Tubulin antibody (10094-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.


Tested Applications

Positive WB detected inU-251 cells, HEK-293 cells, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue, human colon tissue, human cerebellum tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10094-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, fish, hamster, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0136
Full Name tubulin, beta 3
Calculated molecular weight 50 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000748
Gene symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Protocols

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
mouseWB

Cell Metab

Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.

Authors - Marco Rosina
humanWB

Nat Immunol

The tumor suppressor PTEN has a critical role in antiviral innate immunity.

Authors - Shun Li
humanIF

Adv Sci (Weinh)

The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.

Authors - Qixue Wang
mouseWB

Mol Cell

HRD1-mediated METTL14 degradation regulates m6A mRNA modification to suppress ER proteotoxic liver disease.

Authors - Juncheng Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susanne (Verified Customer) (11-25-2022)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 : 1000
  • Cell Tissue Type: human dermal fibroblasts (lysates)
Beta Tubulin Antibody Western Blot validation (1 : 1000 dilution) in human dermal fibroblasts (lysates) (Cat no:10094-1-AP)
FH

Macarena (Verified Customer) (10-07-2022)

excellent results. No non specific binding. The loading control of choice in my lab

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: cell lines
FH

parisa (Verified Customer) (12-03-2021)

We had the anti-mouse antibody and tried this anti-rabbit antibody which worked very well and will be helpful for us.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293T
FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Beta Tubulin Polyclonal Antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mice spinal cord tissues
FH

Elise (Verified Customer) (12-27-2020)

Works perfectly well!

  • Applications: Western Blot
  • Cell Tissue Type: Human spermatozoa