"Beta Tubulin Antibodies" Comparison
View side-by-side comparison of Beta Tubulin antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in
|U-251 cells, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse brain tissue, rat brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|rat brain tissue, mouse brain tissue, human colon tissue, human cerebellum tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10094-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, goat, chicken, sheep, fish, zebrafish, hamster, pig
|Host / Isotype
|Rabbit / IgG
|Beta Tubulin fusion protein Ag0136
|tubulin, beta 3
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
|Product Specific Protocols
|WB protocol for Beta Tubulin antibody 10094-1-AP
|IHC protocol for Beta Tubulin antibody 10094-1-AP
|IF protocol for Beta Tubulin antibody 10094-1-AP
|IP protocol for Beta Tubulin antibody 10094-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susanne (Verified Customer) (11-25-2022)
Macarena (Verified Customer) (10-07-2022)
excellent results. No non specific binding. The loading control of choice in my lab
parisa (Verified Customer) (12-03-2021)
We had the anti-mouse antibody and tried this anti-rabbit antibody which worked very well and will be helpful for us.
Azita (Verified Customer) (06-02-2021)
Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Beta Tubulin Polyclonal Antibody at dilution of 1:50 showed strong labelling.
Elise (Verified Customer) (12-27-2020)
Works perfectly well!