Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 17 publications below |
| IHC | See 13 publications below |
| IF | See 11 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
10593-1-AP targets CD138/Syndecan-1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0823 Product name: Recombinant human SDC1,CD138 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC008765 Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA Predict reactive species |
| Full Name | syndecan 1 |
| Calculated Molecular Weight | 32.5 kDa |
| Observed Molecular Weight | 60-100 kDa, 130 kDa |
| GenBank Accession Number | BC008765 |
| Gene Symbol | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_2182736 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD138/Syndecan-1 antibody 10593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Theranostics Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production. | ||
Mol Neurodegener Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model. | ||
Cell Mol Life Sci The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium. |
