AUH Polyclonal antibody

AUH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17079-1-AP

Synonyms

AUH



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

RIPSee 1 publications below

Product Information

17079-1-AP targets AUH in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AUH fusion protein Ag11265
Full Name AU RNA binding protein/enoyl-Coenzyme A hydratase
Calculated Molecular Weight 310 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC020722
Gene Symbol AUH
Gene ID (NCBI) 549
RRIDAB_2061316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AUH antibody 17079-1-APDownload protocol
IHC protocol for AUH antibody 17079-1-APDownload protocol
IP protocol for AUH antibody 17079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanRIP

Int J Biol Macromol

m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway

Authors - Aini Pan