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Tested Applications
| Positive WB detected in | mouse liver tissue, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
20363-1-AP targets UBN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ubinuclein 1 |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 120-140 kDa |
| GenBank Accession Number | NM_016936 |
| Gene Symbol | UBN1 |
| Gene ID (NCBI) | 29855 |
| RRID | AB_10694818 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPG3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBN1-Specific antibody 20363-1-AP | Download protocol |
| WB protocol for UBN1-Specific antibody 20363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
