Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 24 publications below |
| CoIP | See 1 publications below |
Product Information
27921-1-AP targets PI3 Kinase p110 Alpha in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27539 Product name: Recombinant human PIK3CA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 345-550 aa of BC113601 Sequence: NVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDF Predict reactive species |
| Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| Calculated Molecular Weight | 1068 aa, 124 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC113601 |
| Gene Symbol | PIK3CA |
| Gene ID (NCBI) | 5290 |
| RRID | AB_2918135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42336 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Alpha antibody 27921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology. | ||
Fitoterapia Integrating LC-MS and molecular docking technology to investigate the effect of Spatholobi caulis flavonoids in alleviating primary dysmenorrhea via regulating the RhoA/ROCK pathway | ||
Heliyon Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibition | ||
Pharmaceuticals (Basel) Revealing the Mechanism of Hemerocallis citrina Baroni in Depression Treatment Through Integrated Network Pharmacology and Transcriptomic Analysis | ||
Cardiovasc Pathol Circ_0005372 targets the miR-153-3p/ITGB3 axis to stimulate the PI3K/AKT signaling pathway to facilitate the occurrence and development of congenital heart disease and pulmonary arterial hypertension in children | ||
Elife A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression |
