Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
30255-1-AP targets PSD95 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33000 Product name: Recombinant human PSD95 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 630-724 aa of NM_001365 Sequence: VSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL Predict reactive species |
| Full Name | discs, large homolog 4 (Drosophila) |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 90-95 kDa |
| GenBank Accession Number | NM_001365 |
| Gene Symbol | PSD95 |
| Gene ID (NCBI) | 1742 |
| RRID | AB_3086283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSD95 antibody 30255-1-AP | Download protocol |
| IHC protocol for PSD95 antibody 30255-1-AP | Download protocol |
| WB protocol for PSD95 antibody 30255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Lancao decoction alleviates Alzheimer's disease: depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus | ||
FASEB J HDAC1/2/3-mediated downregulation of neurogranin is involved in cognitive impairment in offspring exposed to maternal subclinical hypothyroidism | ||
ACS Chem Neurosci Nomilin Alleviates Neuroinflammation and Depressive-like Behaviors in Mice through the Upregulation of TAX1BP1-Mediated Modulation of Microglia Polarization | ||
Actas Esp Psiquiatr Exercise-induced Activation of SIRT1/BDNF/mTORC1 Signaling Pathway: A Novel Mechanism to Reduce Neuroinflammation and Improve Post-stroke Depression | ||
Biochem Biophys Res Commun Protective role of TRPV2 in synaptic plasticity through the ERK1/2-CREB-BDNF pathway in chronic unpredictable mild stress rats | ||
Aging (Albany NY) Chronic stress induces Alzheimer's disease-like pathologies through DNA damage-Chk1-CIP2A signaling |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Olga (Verified Customer) (10-03-2025) | You can surely dilute it more than 1:1000, as the signal was already saturated at 2.5 seconds of exposition. I find it better for mouse samples as it does not give non-specificity in non-brain tissues (comparing to 20665-1-AP PSD95 antibody) - all the mouse cells tested are of neuronal origin. The principal band size depends on protein marker used (see picture) - and can vary from 80 to 110 kDa.
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FH Alessandro (Verified Customer) (02-04-2025) | high specificity and sensitivity, providing reliable and reproducible results
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