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  • Western Blot (WB) analysis of various lysates using STAP2 Polyclonal antibody (30516-1-AP)

    WB analysis using 30516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STAP2 Polyclonal antibody (30516-1-AP)

    WB analysis using 30516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

STAP2 Polyclonal antibody

STAP2 Polyclonal Antibody for WB, ELISA

Cat No. 30516-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BKS, Breast tumor kinase substrate, BRK substrate, Signal-transducing adaptor protein 2, STAP-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30516-1-AP targets STAP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAP2 fusion protein Ag28818 Predict reactive species
Full Name signal transducing adaptor family member 2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000795
Gene Symbol STAP2
Gene ID (NCBI) 55620
RRIDAB_3086344
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAP2, also known as BKS, belongs to the signal-transducing adaptor protein (STAP) family, The family includes STAP1 and STAP2, is a group of adaptor molecules involved in regulating multiple signaling pathways. The expression level of STAP2 in tubular cells is significantly higher than that in other renal cells, accounting for approximately 70%. In tubular epithelial cells, which are primarily affected by renal injury, STAP2 is mainly localized in the cytoplasm (PMID: 39533293).

Protocols

Product Specific Protocols
WB protocol for STAP2 antibody 30516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols