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  • Western Blot (WB) analysis of various lysates using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis using 67952-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of HSC-T6 using 67952-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of 4T1 using 67952-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of human placenta using 67952-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using TrpRS Monoclonal antibody (67952-1-Ig)

    IF Staining of LNCaP using 67952-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67952-1-Ig
Clone No.1C6C3

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, 4T1 cells, human placenta tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67952-1-Ig targets TrpRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag31430

Product name: Recombinant human WARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

Predict reactive species
Full Name tryptophanyl-tRNA synthetase
Calculated Molecular Weight 471 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017489
Gene Symbol WARS1
Gene ID (NCBI) 7453
RRIDAB_2918704
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

Protocols

Product Specific Protocols
IF protocol for TrpRS antibody 67952-1-IgDownload protocol
WB protocol for TrpRS antibody 67952-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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