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Tested Applications
| Positive WB detected in | rat heart tissue, rabbit heart tissue, LNCaP cells, mouse heart tissue, mouse liver tissue, rat liver tissue, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
68128-1-Ig targets ATP5J2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8811 Product name: Recombinant human ATP5J2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-94 aa of BC003678 Sequence: MASVGECPAPVPVKDKKLLEVKLLELPSWILMRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFSYSFSYKHLKHERLRKYH Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2 |
| Calculated Molecular Weight | 5-11 kDa |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC003678 |
| Gene Symbol | ATP5J2 |
| Gene ID (NCBI) | 9551 |
| RRID | AB_2923655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P56134 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP5J2 antibody 68128-1-Ig | Download protocol |
| WB protocol for ATP5J2 antibody 68128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration | ||
J Transl Med Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC | ||
