Tested Applications
| Positive WB detected in | U2OS cells, T-47D cells, MDA-MB-231 cells, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68361-1-Ig targets WSTF in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24403 Product name: Recombinant human BAZ1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1144-1483 aa of BC136520 Sequence: KVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK Predict reactive species |
| Full Name | bromodomain adjacent to zinc finger domain, 1B |
| Calculated Molecular Weight | 1483 aa, 171 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC136520 |
| Gene Symbol | WSTF/BAZ1B |
| Gene ID (NCBI) | 9031 |
| RRID | AB_3085083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UIG0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tyrosine-protein kinase BAZ1B, also named WSTF, plays a central role in chromatin remodeling and acts as a transcription regulator (PMID:19092802). It involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph) (PMID:19234442).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WSTF antibody 68361-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
