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  • Western Blot (WB) analysis of various lysates using MCM3 Recombinant antibody (84792-2-RR)

    WB analysis using 84792-2-RR (same clone as 84792-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-2-RR (MCM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MCM3 Recombinant antibody (84792-2-RR)

    WB analysis of HeLa using 84792-2-RR (same clone as 84792-2-PBS)

    WB result of MCM3 antibody (84792-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM3 transfected HeLa cells. This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using MCM3 Recombinant antibody (84792-2-RR)

    IF Staining of Raji using 84792-2-RR (same clone as 84792-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using MCM3 antibody (84792-2-RR, Clone: 242103E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using MCM3 Recombinant antibody (84792-2-RR)

    FC experiment of K-562 using 84792-2-RR (same clone as 84792-2-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mcm3 Recombinant Antibody (84792-2-RR, Clone:242103E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MCM3 Recombinant antibody (84792-2-RR)

    FC experiment of HeLa using 84792-2-RR (same clone as 84792-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mcm3 Recombinant Antibody (84792-2-RR, Clone:242103E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84792-2-PBS

    Affinity and Kinetic Characterization of 84792-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84792-2-PBS against Human MCM3 was performed. The affinity constant is 3.09 nM.

  • Featured Product
  • KD/KO Validated

MCM3 Recombinant monoclonal antibody, PBS Only

MCM3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84792-2-PBS
Clone No.242103E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242103E4, DNA polymerase alpha holoenzyme-associated protein P1, EC:3.6.4.12, P1 MCM3, p102

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

84792-2-PBS targets MCM3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7966

Product name: Recombinant human MCM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-503 aa of BC001626

Sequence: IQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLR

Predict reactive species
Full Name minichromosome maintenance complex component 3
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 102-105 kDa
GenBank Accession NumberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25205
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

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