Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, A431 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84873-4-RR targets HSPA1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HSPA1B fusion protein Ag13700 Predict reactive species |
| Full Name | heat shock 70kDa protein 1B |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC057397 |
| Gene Symbol | HSP70 |
| Gene ID (NCBI) | 3304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08107 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSP70 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSP70 is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Recently it has been reported that HSP70 can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA1B antibody 84873-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
