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  • Western Blot (WB) analysis of various lysates using CCR3 Recombinant antibody (86038-1-RR)

    WB analysis using 86038-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86038-1-RR (CCR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CCR3 Recombinant antibody (86038-1-RR)

    IF Staining of K-562 using 86038-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCR3 antibody (86038-1-RR, Clone: 250209D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCR3 Recombinant antibody

CCR3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86038-1-RR
Clone No.250209D5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C C chemokine receptor type 3, C C CKR 3, C C CKR3, C-C chemokine receptor type 3, CC CKR 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA375 cells, THP-1 cells, Jurkat cells, HUVEC cells, U-87 MG cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86038-1-RR targets CCR3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name chemokine (C-C motif) receptor 3
Calculated Molecular Weight 355 aa, 41 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC110297
Gene Symbol CCR3
Gene ID (NCBI) 1232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR3 (C-C Motif Chemokine Receptor 3), also known as CD193, is a G protein-coupled receptor (GPCR) primarily involved in immune cell trafficking and inflammation regulation and highly expressed in eosinophils and basophils, with detectable levels in Th2 cells, airway epithelial cells, and subsets of monocyte-derived dendritic cells (PMID: 21077277). Ligand-induced CCR3 internalization is required for eosinophil shape change and actin polymerization but not for calcium mobilization or desensitization (PMID: 12532103).

Protocols

Product Specific Protocols
WB protocol for CCR3 antibody 86038-1-RRDownload protocol
IF protocol for CCR3 antibody 86038-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols