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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-ATR (Ser428) Recombinant antibody (86330-1-RR)

    WB analysis of HEK-293 using 86330-1-RR

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Phospho-ATR (Ser428) Recombinant monoclonal antibo (86330-1-RR)

    IF Staining of HEK-293 using 86330-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-ATR (Ser428) Recombinant monoclonal antibody

Phospho-ATR (Ser428) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86330-1-RR
Clone No.250988D3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86330-1-RR targets Phospho-ATR (Ser428) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name ataxia telangiectasia and Rad3 related
Calculated Molecular Weight 301 kDa
Observed Molecular Weight300 kDa
GenBank Accession NumberNM_001184
Gene Symbol ATR
Gene ID (NCBI) 545
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13535
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)

Protocols

Product Specific Protocols
WB protocol for Phospho-ATR (Ser428) antibody 86330-1-RRDownload protocol
IF protocol for Phospho-ATR (Ser428) antibody 86330-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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