Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, EC109 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86974-2-RR targets HP1BP3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag18709 Product name: Recombinant human HP1BP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-302 aa of BC045660 Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK Predict reactive species |
| Full Name | heterochromatin protein 1, binding protein 3 |
| Calculated Molecular Weight | 553 aa, 61 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC045660 |
| Gene Symbol | HP1BP3 |
| Gene ID (NCBI) | 50809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5SSJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HP1BP3 (heterochromatin protein 1-binding protein 3) is an evolutionarily conserved nuclear protein that functions as a non-canonical linker histone. It is broadly expressed in most vertebrate tissues and is structurally related to the histone H1 family, containing three globular domains and a highly basic C-terminal tail reminiscent of H1 proteins . Unique to HP1BP3 are an acidic N-terminal stretch and a consensus HP1-binding motif (PxVxL), through which it associates with heterochromatin protein 1 (HP1α/β) and is enriched at perinucleolar heterochromatin regions marked by H3K9me3. Functionally, HP1BP3 acts as a modulator of gene expression and genome stability. Alternative splicing was reported to produce 4 different isoforms of HP1BP3, the calculated MW corresponding to 61, 57, 44, 15 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HP1BP3 antibody 86974-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
