Tested Applications
| Positive WB detected in | HEK-293T cells, human heart tissue, mouse spleen tissue, HepG2 cells, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87058-2-RR targets PDLIM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3934 Product name: Recombinant human PDLIM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC027870 Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA Predict reactive species |
| Full Name | PDZ and LIM domain 3 |
| Calculated Molecular Weight | 316 aa, 34 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC027870 |
| Gene Symbol | PDLIM3 |
| Gene ID (NCBI) | 27295 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q53GG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM3 antibody 87058-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
