Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87431-1-RR targets HMBS in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6509 Product name: Recombinant human HMBS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC000520 Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH Predict reactive species |
| Full Name | hydroxymethylbilane synthase |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC000520 |
| Gene Symbol | HMBS |
| Gene ID (NCBI) | 3145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase (PBGD) or previously as uroporphyrinogen I synthase, is a key enzyme in the heme biosynthesis pathway. HMBS is a cytoplasmic enzyme that catalyzes a critical step in the heme biosynthesis pathway: the polymerization of four porphobilinogen molecules to form linear hydroxymethylbilane. It is expressed in all tissues, with the highest levels observed in the liver and erythroid precursors to meet high heme demand. Two main isoforms exist: a ubiquitous "housekeeping" isoform and an erythroid-specific isoform. The observed molecular weight of human HMBS protein is approximately 40-42 kDa (as determined by SDS-PAGE). The erythroid-specific isoform has a slightly lower molecular weight due to a different translation initiation site. (PMID: 19292878, PMID: 12555854)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HMBS antibody 87431-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
