Tested Applications
| Positive WB detected in | C2C12 cells, mouse stomach tissue, rat stomach tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87433-1-RR targets CD315/PTGFRN in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6319 Product name: Recombinant mouse CD315/PTGFRN protein (C-rFc) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-832 aa of NM_011197.3 Sequence: RVVRVPAGTLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSSGSSFVELASTWEVGFPAQLYRERLQRGDILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADALVVGPSSRPPPGLSLREGEPFELRCIASTTSPLHTHLALRWELHRGPVHRSILALSHEGRFHPGPGYEQRYHSGDVRLDTVGSDAYRLSVARALSADQGSYRCVVSEWITEQGSWQEIQEKAVEVATVVIQPTALQLAVPRTVSVTEGKDLDLSCNITTDRVDDVRPEVTWYFKKTPDTSLLASHMLARLDRDSLVHSSPHVALSHVDTRSYHLLVRDVSKENSGYYLCLVALWAPGHNRSWHKVAEAMSAPSGVSVTWLEPEYQVYLNASKVPGFSDDPTELQCRVIDTKRLEAGVRLTVSWYYRMTRRNDDVVASELLAVMDGDWTLRYGERSKQRAQDGEFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTRQRNNSWVKSKDVFSKPVNIFWASEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLITAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPIGGSLWREAATSLSNPIEIDFQTSGPTFNASVHSDTPSVTRGDLIKLFCIVTVEGAVLDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGVVTTGQRDWKSTVSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVRSPTGSWQREAEIHSRPIFITVKMDVLNAFKYP Predict reactive species |
| Full Name | prostaglandin F2 receptor negative regulator |
| Calculated Molecular Weight | 99 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | NM_011197.3 |
| Gene Symbol | Ptgfrn |
| Gene ID (NCBI) | 19221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9WV91 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD315/PTGFRN antibody 87433-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
