Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87628-1-RR targets ST5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34032 Product name: Recombinant human ST5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10-344 aa of BC036655 Sequence: SITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPIYPLSDSETSACRYPSHSSSRVLLKDRHPPAPSPQNPQDPSPDTSPPTCPFKTASFGYLDRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPFPGPAAGPRGVLLTRTGTRAHSLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVVPSPCSSEKTFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRKESSAVLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRNTGTLGSLEEPAGGASVSAGSRAVGVAGV Predict reactive species |
| Full Name | suppression of tumorigenicity 5 |
| Calculated Molecular Weight | 1137 aa, 126 kDa |
| Observed Molecular Weight | 120-150 kDa |
| GenBank Accession Number | BC036655 |
| Gene Symbol | ST5 |
| Gene ID (NCBI) | 6764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ST5, also known as DEN2B (DENN domain-containing protein 2B), has three isoforms with molecular weights of 126 kDa, 82 kDa, and 70 kDa, respectively. The mainstream molecular weight is 126kd. ST5 is widely expressed except in peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial cells and fibroblasts (including tumorigenic cells), but not in lymphoid cell lines (at the protein level).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST5 antibody 87628-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
