Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87659-1-RR targets GGT1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6301 Product name: Recombinant mouse CD224/GGT1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-568 aa of NM_001305992.1 Sequence: PYTSGKPDHVYSRAAVATDAKRCSEIGRDILQEGGSVVDAAIASLLCMGLMNAHSMGIGGGLFFTIYNSTTGKVEVINAREVAPRLANTTMFNNSKDSEEGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLAIALDKKRDVIEKTPALCEVFCRQGKVLQEGETVTMPKLADTLQILAQEGAKAFYNGSLTAQIVKDIQEAGGIMTVEDLNNYRAELIEHPMSIGLGDATLYVPSAPLSGPVLILILNILKGYNFSPKSVATPEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHPAAYYEPEFYLQDDGGTAHLSAVSEDGSAVAATSTINLYFGSKVLSRVSGILFNDEMDDFSSPNFINQFRVAPSPANFIKPGKQPLSSMCPSIILDKDGQVRMVVGASGGTQITTSVALAIINSLWFGYDVKRAVEEPRLHNQLLPNTTTVEKDIDQVVTAGLKIRHHHTEVTPTFIAVVQAVVRASGGWAAASDSRKGGEPAGY Predict reactive species |
| Full Name | gamma-glutamyltransferase 1 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 50-61 kDa |
| GenBank Accession Number | NM_001305992.1 |
| Gene Symbol | Ggt1 |
| Gene ID (NCBI) | 14598 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q60928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GGT1 is a heterodimer composed of light chain and heavy chain, and its active site is located in the light chain. The metabolism of glutathione by GGT1 releases free glutamic acid and dipeptide cysteine-glycine, which can be hydrolyzed into cysteine and glycine by dipeptidase. In the presence of high concentration of dipeptide and some amino acids, it can also catalyze the peptide transfer reaction and transfer γ -glutamyl group to receptor amino acids to form new γ -glutamyl compounds. Participate in cysteine homeostasis, glutathione homeostasis and the transformation of leukotriene LTC4 to LTD4 (PMID:12810527).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GGT1 antibody 87659-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
