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Recombinant Human IFN-gamma protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0004

Validation Data Gallery

  • Purity of Recombinant Human IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IFN-gamma protein Gln24-Gly161 (Accession# P01579) with a Myc tag and a His tag at the C-terminus.
GeneID 3458
Accession P01579
PredictedSize 21.2 KDa
SDS-PAGE 25-38 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interferon gamma (IFN γ) is a type II interferon that confers immunity to bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types, including natural killer cells, natural killer T cells and effector lymphocyte T cells, in response to antigenic and inflammatory stimuli. The IFN-γ dimer binds to its cognate receptor, which has two subunits: IFN-γR1, the ligand binding chain (α chain), and IFN-γR2, the signal transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway, which in turn activates IFN-γ responsive genes. While IFN γ can inhibit viral replication, it also acts as an immunomodulator and immunostimulator by increasing the surface expression of class I MHC proteins.

References:

1.Billiau, Alfons et al. Cytokine & growth factor reviews vol. 20,2 (2009): 97-113. 
2.Tau, G et al. Allergy vol. 54,12 (1999): 1233-51.
3.Ding, Huihui et al. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie vol. 155, (2022): 113683.
4.Cordeiro, Patrícia Azevedo Soares et al. Revista do Instituto de Medicina Tropical de Sao Paulo vol. 64, (2022): e64.