• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human CEACAM-6/CD66c protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 12-46 ng/mL

Cat no : Eg0056

Validation Data Gallery

  • Purity of Recombinant Human CEACAM-6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM-6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 12-46 ng/mL.

    Immobilized Human CEACAM-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 12-46 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human CEACAM-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 12-46 ng/mL.
Expression HEK293-derived Human CEACAM-6 protein Lys35 -Gly320 (Accession# P40199) with a Myc tag and a His tag at the C-terminus.
GeneID 4680
Accession P40199
PredictedSize 36.3 kDa
SDS-PAGE 40-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), belonging to the immunoglobulin superfamily, is cell-adhesion protein on neutrophils. CEACAM6 is expressed in neutrophils and numerous tumor cell lines. CEACAM6 mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. It plays a role in neutrophil adhesion to cytokine-activated endothelial cells and plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion. CEACAM6 is also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells.

References:

1.Kuroki M, et al. (2001). Journal of leukocyte biology. 70(4): 543-50 
2.Kuijpers TW, et al. (1992). J Cell Biol. 118(2):457-66
3.Blumenthal RD, et al. (2005). Cancer research. 65(19):8809–8817
4.Ordoñez C, et al. (2000). Cancer Res. 60(13):3419-24
5.Duxbury MS, et al. (2004). Oncogene. 23(2):465-73