• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human CD28 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0889

Validation Data Gallery

  • Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD28 protein Asn19-Pro152 (Accession# P10747-1) with a His tag at the C-terminus.
GeneID 940
Accession P10747-1
PredictedSize 16.2 kDa
SDS-PAGE 30-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD28 (T-cell-specific surface glycoprotein CD28), or T44 and Tp44, is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 may affect in vivo immune responses as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway. CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop. In addition, CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death.

References:

1. C H June. et al. (1990). Immunol Today. 11(6):211-216.
2. P S Linsley. et al. (1993). Annu Rev Immunol. 11:191-212.
3. Edward J Evans. et al. (2005). Nat Immunol. 6(3):271-279.
4. W Lesslauer. et al. (1986). Eur J Immunol. 16(10):1289-1296
5. R J Peach. et al. (1994). J Exp Med. 180(6):2049-2058.