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Recombinant Mouse TIGIT protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 16-65 ng/mL

Cat no : Eg0925

Validation Data Gallery

  • Purity of Recombinant Mouse TIGIT was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse TIGIT was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.

    Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.
Expression HEK293-derived Mouse TIGIT protein Gly26-Thr143 (Accession# NP_001139797.1) with a His tag at the C-terminus.
GeneID 100043314
Accession NP_001139797.1
PredictedSize 13.7 kDa
SDS-PAGE 15-25 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells. It contains an immunoglobulin variable domain, a transmembrane domain, and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM.

References:

1.Xin Yu, et al. (2009). Nat Immunol. 10(1):48-57.
2.Noa Stanietsky, et al. (2009). Proc Natl Acad Sci U S A. 106(42):17858-63. 
3.Joe-Marc Chauvin, et al. (2020). J Immunother Cancer. 8(2):e000957.