Recombinant Mouse Endoglin/CD105 protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg1292
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Endoglin/CD105 protein Glu27-Lys580 (Accession# Q63961) with a His tag at the C-terminus. |
| GeneID | 13805 |
| Accession | Q63961 |
| PredictedSize | 60.9 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis.
References:
1. Cheifetz, S et al. The Journal of biological chemistry vol. 267,27 (1992): 19027-30. 2. Fonsatti, Ester et al. Oncogene vol. 22,42 (2003): 6557-63. 3. Nassiri, Farshad et al. Anticancer research vol. 31,6 (2011): 2283-90. 4. Duff, Sarah E et al. FASEB journal vol. 17,9 (2003): 984-92.