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Recombinant Human IL-5RA/CD125protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1665

Validation Data Gallery

  • Purity of Recombinant Human IL-5RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-5RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IL-5RA protein Asp21-Glu335 (Accession# Q01344-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 3568
Accession Q01344-1
PredictedSize 62.1 kDa
SDS-PAGE 65-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-5RA, also known as the interleukin-5 receptor subunit alpha (CD125), is a crucial component of the interleukin-5 receptor complex, which plays a significant role in immune responses. IL-5RA is a specific subunit of a heterodimeric cytokine receptor that, along with the signal transducing beta subunit (IL-5RB), forms the complete IL-5 receptor complex. IL-5RA is primarily expressed on the surface of eosinophils and is responsible for the proliferation, differentiation, and activation of these cells. It is a key regulator in allergic reactions and asthma (PMID: 21986312; 28913336; 18511250).

References:

1. Takatsu K. et al. (2011). Proc Jpn Acad Ser B Phys Biol Sci. 87(8):463-485.
2. Bagnasco D. et al. (2017). Front Med (Lausanne). 4:135.
3. Takatsu K. et al. (2008). Curr Opin Immunol. 20(3):288-294.