Recombinant Mouse CRTAM/CD355 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2691
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CRTAM protein Ala17-Ala284 (Accession# Q149L7-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 54698 |
| Accession | Q149L7-1 |
| PredictedSize | 55.5 kDa |
| SDS-PAGE | 65-85 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion. CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes. It also regulates CD8+ T cell retention within the lymph node and eventually effector function.
References:
1.Yeh JH, et al. (2008) Cell. 7;132(5):846-59. 2.Garay E, et al. (2010) J Cell Biochem. 1;111(1):111-22. 3.Takeuchi A, et al. (2015) J Exp Med. 11;213(1):123-38. 4.Takeuchi A,et al. (2009) J Immunol. 1;183(7):4220-8.