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Recombinant Human CD62L protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3006

Validation Data Gallery

  • Purity of Recombinant Human CD62L was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD62L was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD62L protein Trp39-Asn332 (Accession# P14151-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 6402
Accession P14151-1
PredictedSize 59.1 kDa
SDS-PAGE 68-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes. CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes. It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation.

References:

1.Bowen BR. et al. (1989) J Cell Biol. 109(1):421-427.
2.Tedder TF. et al. (1989) J Exp Med. 170(1):123-133.
3.Griffin JD. et al. (1990) J Immunol. 145(2):576-584.
4.Tedder TF. et al. (1990) Eur J Immunol. 20(6):1351-1355. 
5.Tedder TF. et al. (1990) J Immunol. 144(2):532-540.
6.Schleiffenbaum B. et al. (1992) J Cell Biol. 119(1):229-238.