Recombinant Human Complement Factor I protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3066
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Complement Factor I protein Lys19-Val583 (Accession# P05156) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3426 |
| Accession | P05156 |
| PredictedSize | 89.1 kDa |
| SDS-PAGE | 95-120 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 30 mM Tris, 0.5 M Arg, pH 8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Complement Factor I (CFI) protein is a key plasma serine protease, which is mainly synthesized in the liver, with a molecular weight of about 75-80 kDa, and circulates in the blood in the form of heterodimers of heavy chain (51 kDa)- light chain (37 kDa) disulfide bonds.
References:
1. Lachmann, Peter J. Immunobiology vol. 224,4 (2019): 511-517. 2. Zhang, Yuzhou et al. Frontiers in immunology vol. 13 866330. 10 May. 2022