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Recombinant Human HBEGF protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3862

Validation Data Gallery

  • Purity of Recombinant Human HBEGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) condition and stained using Coomassie blue.

    Purity of Recombinant Human HBEGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) condition and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human HBEGF protein Leu20-Thr160 (Accession# Q99075) with a rabbit IgG Fc tag at the C-terminus.
GeneID 1839
Accession Q99075
PredictedSize 42.0 kDa
SDS-PAGE 13-17 kDa & 42-54 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The epidermal growth factor (EGF) family member, heparin-binding EGF-like growth factor, (HBEGF), is present in the uterus at the time of embryo implantation, and its expression in trophoblast (TB) cells of the placenta indicate its central role in early implantation and subsequent placentation. HBEGF protects TB cells from apoptosis, and promotes their invasion. Transmembrane HBEGF cleavage in response to low O2 initiates autocrine signaling that increases HBEGF levels to a concentration that inhibits apoptosis. The autocrine upregulation of HBEGF requires activation of its cognate receptors (ERBB1 and ERBB4), and downstream signaling through any one of three MAPK pathways initiated by MAPK 1/3, MAPK14 or MAPK8.

References:

1. Yoo, H J et al. Developmental genetics vol. 21,1 (1997): 102-8. 
2. Leach, R E et al. The Journal of clinical endocrinology and metabolism vol. 84,9 (1999): 3355-63. 
3. Armant, D Randall et al. Development (Cambridge, England) vol. 133,4 (2006): 751-9. 
4. Jain, Chandni V et al. PloS one vol. 11,10 (2016) e0163913. 
5. Jessmon, Philip et al. Biology of reproduction vol. 82,5 (2010): 921-9.