Recombinant Human PIGR protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6497
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human PIGR protein Lys19-Arg638 (Accession# P01833) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 5284 |
| Accession | P01833 |
| PredictedSize | 93.4 kDa |
| SDS-PAGE | 90-125 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
The polymeric immunoglobulin receptor (PIGR), an integral membrane protein, is required for transcytosis of polymeric immunoglobulins (pIg) including IgA and IgM across mucosal epithelial cells. PIGR is expressed on the basolateral surface of ciliated epithelial cells in the mucosal epithelium. Upregulation of pIgR expression has been well documented with a variety of proinflammatory cytokines such as IFN-γ and IL-1α.
References:
1. Zhang, J R et al. Cell vol. 102,6 (2000): 827-37. 2. Turula H, Wobus CE. Viruses. (2018);10(5):237. 3. Wei H, Wang JY. Int J Mol Sci. (2021);22(5):2284.