Recombinant Mouse Vitamin D binding protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg7266
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Vitamin D binding protein Leu17-Ser476 (Accession# P21614) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 14473 |
| Accession | P21614 |
| PredictedSize | 77.9 kDa |
| SDS-PAGE | 70-87 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Vitamin D binding protein (DBP), also known as group-specific component (GC-globulin), is a glycosylated alpha-globulin produced primarily by the liver. It is a multifunctional, highly expressed, polymorphic serum protein. And it is involved in vitamin D transport and storage, scavenges of extracellular G-actin, and enhances of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. The presence of genetic variants in the Vitamin D BP gene are related to certain diseases, mostly concerning cancers (breast, prostate, pancreatic, lung, colorectal, basal cell carcinoma cancer and cutaneous melanoma) or other related diseases (thyroid autoimmunity disorders, obesity, diabetes mellitus, bone metabolism, rheumatoid arthritis, ankylosing spondylitis, asthma, chronic obstructive pulmonary disease, tuberculosis and coronary artery diseases).
References:
1.White, P, and N Cooke. Trends in endocrinology and metabolism: TEM vol. 11,8 (2000): 320-7. 2.Bhan, Ishir. International journal of endocrinology vol. 2014 (2014): 561214. 3.Nagasawa, Hideko et al. Anticancer research vol. 25,6A (2005): 3689-95. 4.Rozmus, Dominika et al. International journal of molecular sciences vol. 21,21 7822. 22 Oct. 2020, doi:10.3390/ijms21217822.