ABCB5 Polyclonal antibody

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  WB analysis using 20703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20703-1-AP (ABCB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ABCB5 Polyclonal Antibody for WB, ELISA

Cat No. 20703-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P-glycoprotein ABCB5, EC:7.6.2.2, ATP-binding cassette sub-family B member 5, ABCB5beta, ABCB5alpha

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Tested Applications

• Positive WB detected in: A431 cells, HT-29 cells, Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20703-1-AP targets ABCB5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: NM_178559
• Gene Symbol: ABCB5
• Gene ID (NCBI): 340273
• RRID: AB_3085616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2M3G0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCB5 is an 812 amino acid multi-pass membrane protein and belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins (PMID: 15760339). ABCB5 is an Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells(PMID: 15899824, 15205344). ABCB5 acts as a marker of stem-like cells (CSC) in several malignancies and is responsible for the resistance to doxorubicin of a subset of malignant melanomas (PubMed:24934811, 15760339). Alternate splicing results in multiple transcript variants.