ABHD6 Polyclonal antibody

ABHD6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20494-1-AP

Synonyms

ABHD6, Monoacylglycerol lipase ABHD6

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 20494-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 20494-1-AP is ABHD6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD6 fusion protein Ag13968
• Full Name: abhydrolase domain containing 6
• Calculated molecular weight: 337 aa, 38 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC001698
• Gene symbol: ABHD6
• Gene ID (NCBI): 57406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Neuron; An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.; Authors - Jochen Schwenk
mouse	WB	Pharmacol Res; Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice; Authors - Zhiwei Xue