ABHD6 Polyclonal antibody

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  WB analysis using 20494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ABHD6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20494-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2-arachidonoylglycerol hydrolase, ABHD 6, Abhydrolase domain-containing protein 6, EC:3.1.1.23

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Tested Applications

• Positive WB detected in: rat spleen tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

20494-1-AP targets ABHD6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD6 fusion protein Ag13968
• Full Name: abhydrolase domain containing 6
• Calculated Molecular Weight: 337 aa, 38 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC001698
• Gene Symbol: ABHD6
• Gene ID (NCBI): 57406
• RRID: AB_2878694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BV23
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

α/β-hydrolase domain 6 (ABHD6) is a lipase linked to physiological functions affecting energy metabolism. ABHD6, which was first identified in the brain ( PMID: 18096503) , is a ubiquitously expressed lipase.

Publications

Species	Application	Title
mouse	WB	Neuron; An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.; Authors - Jochen Schwenk
mouse	WB	Pharmacol Res; Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice; Authors - Zhiwei Xue
human	WB	Mol Carcinog; ABHD6 suppresses colorectal cancer progression via AKT signaling pathway; Authors - Xiaoyu Xiong ; KD Validated