ABI2 Polyclonal antibody

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  WB analysis using 14890-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14890-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 14890-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14890-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14890-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

ABI2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14890-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6679

  Product name: Recombinant human ABI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-275 aa of BC001439

  Sequence: MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSV

• Full Name: abl interactor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC001439
• Gene Symbol: ABI2
• Gene ID (NCBI): 10152
• RRID: AB_2288997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Nat Commun; GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis; Authors - Qiang Zhu
mouse,human	WB	Nat Commun; hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS); Authors - Hannah E Salapa
human	WB	Nat Cell Biol; Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis; Authors - Xiaoguang Liu