ABT1 Polyclonal antibody, PBS Only

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  WB analysis of mouse liver using 14148-1-AP (same clone as 14148-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14148-1-AP (same clone as 14148-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 14148-1-AP (same clone as 14148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 14148-1-AP (same clone as 14148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14148-1-AP (same clone as 14148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABT1 antibody (14148-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14148-1-PBS in a different storage buffer formulation.

ABT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14148-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hABT1, Esf2, Activator of basal transcription 1

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Product Information

14148-1-PBS targets ABT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5317

  Product name: Recombinant human ABT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-272 aa of BC066313

  Sequence: MEAEESEKAATEQEPLEGTEQTLDAEEEQEESEEAACGSKKRVVPGIVYLGHIPPRFRPLHVRNLLSAYGEVGRVFFQAEDRFVRRKKKAAAAAGGKKRSYTKDYTEGWVEFRDKRIAKRVAASLHNTPMGARRRSPFRYDLWNLKYLHRFTWSHLSEHLAFERQVRRQRLRAEVAQAKRETDFYLQSVERGQRFLAADGDPARPDGSWTFAQRPTEQELRARKAARPGGRERARLATAQDKARSNKGLLARIFGAPPPSESMEGPSLVRDS

• Full Name: activator of basal transcription 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31-32 kDa
• GenBank Accession Number: BC066313
• Gene Symbol: ABT1
• Gene ID (NCBI): 29777
• RRID: AB_10640440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULW3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.