ACE2 Monoclonal antibody

ACE2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2F12A4

Cat no : 66699-1-Ig

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15



Tested Applications

Positive WB detected inhuman testis tissue, human skeletal muscle tissue
Positive IHC detected inhuman small intestine tissue, human testis tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue, mouse kidney tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66699-1-Ig targets ACE2 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACE2 fusion protein Ag15554
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805 aa, 92 kDa
Observed molecular weight 120 kDa, 92 kDa
GenBank accession numberBC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Protocols

Product Specific Protocols
WB protocol for ACE2 antibody 66699-1-IgDownload protocol
IHC protocol for ACE2 antibody 66699-1-IgDownload protocol
IF protocol for ACE2 antibody 66699-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

medRxiv

Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 sera.

Authors - Kasopefoluwa Y Oguntuyo
humanWB

Res Sq

Evidence that Maackia amurensis seed lectin (MASL) exerts pleiotropic actions on oral squamous cells to inhibit SARS-CoV-2 infection and COVID-19 disease progression.

Authors - Stephanie A Sheehan
humanWB, IF, FC

medRxiv

Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis.

Authors - Ni Huang
humanIF

Cell Stem Cell

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Authors - Ryan M Samuel
humanWB

bioRxiv

SARS-CoV-2 spike downregulates tetherin to enhance viral spread.

Authors - H Stewart
humanIHC

J Laryngol Otol

Differential expression of angiotensin-converting enzyme-2 in human paranasal sinus mucosa in patients with chronic rhinosinusitis.

Authors - T Kawasumi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lindsey (Verified Customer) (05-24-2021)

Great antibody---looking forward to trying the RabPolyAb as well

  • Applications: Immunohistochemistry,Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human lung (FFPE); mouse lung (OCT/frozen)
ACE2 Antibody Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human lung (FFPE); mouse lung (OCT/frozen) (Cat no:66699-1-Ig)
FH

Lindsey (Verified Customer) (09-21-2020)

Perfomed IF analysis on human FFPE and mouse frozen tissue sections; for FFPE tissue, antigen retrieval was performed for 30 min at 95C using Tris EDTA Buffer (pH9.0); Permeabilization was not performed; Blocking was performed using 5% Normal Serum and 0.3% TritonX-100 for 30. minutes at room temperature. Mouse lung sections were costained with ARL13B (Proteintech #1711-1-AP) to visualize primary cilia of the lung epithelia; all sections were counterstained with DAPI Fluormount (Abcam); image attached is of mouse lung frozen tissue; due to size restrictions I am limited to this image only but am happy to provide additional images (human tissue etc.) upon request

  • Applications: Immunohistochemistry,Immunofluorescence,Other
  • Primary Antibody Dilution: 1:1000 (works best for IF with FFPE @ 1:500)
  • Cell Tissue Type: Human lung (FFPE); mouse lung (OCT/frozen)
ACE2 Antibody Immunohistochemistry,Immunofluorescence,Other validation (1:1000 (works best for IF with FFPE @ 1:500) dilution) in Human lung (FFPE); mouse lung (OCT/frozen) (Cat no:66699-1-Ig)