ACE2 Monoclonal antibody

• Featured Product
• KD/KO Validated

ACE2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

23

CloneNo.

2F12A4

Reviews (2)

Cat no : 66699-1-Ig

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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Validation Data Gallery

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  WB analysis of human testis using 66699-1-Ig

  Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis of human testis using 66699-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 66699-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens)..

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  IHC staining of human small intestine using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens)..

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  IHC staining of human kidney using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66699-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 66699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human kidney using 66699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 66699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 66699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66699-1-Ig

  1X10^6 HeLa cells were surface stained with 0.20 ug/test Anti-Human ACE2 (66699-1-Ig, Clone:2F12A4) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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  FC experiment of THP-1 using 66699-1-Ig

  1X10^6 THP-1 cells were surface stained with 0.20 ug/test Anti-Human ACE2 (66699-1-Ig, Clone:2F12A4) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: human testis tissue, human skeletal muscle tissue
• Positive IHC detected in: human small intestine tissue, human testis tissue, human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human kidney tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Product Information

66699-1-Ig targets ACE2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACE2 fusion protein Ag15554
• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated molecular weight: 805 aa, 92 kDa
• Observed molecular weight: 120 kDa, 92 kDa
• GenBank accession number: BC048094
• Gene symbol: ACE2
• Gene ID (NCBI): 59272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Publications

Species	Application	Title
human	WB, IF	Science; Neuropilin-1 is a host factor for SARS-CoV-2 infection.; Authors - James L Daly
human	IF	Nat Med; SARS-CoV-2 infection of the oral cavity and saliva.; Authors - Ni Huang
human	IHC	Ocul Surf; SARS-CoV-2 receptor ACE2 is expressed in human conjunctival tissue, especially in diseased conjunctival tissue.; Authors - Shengjie Li
human	WB	Cell Death Differ; SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination.; Authors - Zhengrong Zhang
mouse	WB	J Control Release; Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections.; Authors - Yizhuo Wang
human	WB	PLoS Pathog; Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways.; Authors - Nicolás Sarute

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lindsey (Verified Customer) (05-24-2021)

Great antibody---looking forward to trying the RabPolyAb as well

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human lung (FFPE); mouse lung (OCT/frozen)

ACE2 Antibody Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human lung (FFPE); mouse lung (OCT/frozen) (Cat no:66699-1-Ig)

FH

Lindsey (Verified Customer) (09-21-2020)

Perfomed IF analysis on human FFPE and mouse frozen tissue sections; for FFPE tissue, antigen retrieval was performed for 30 min at 95C using Tris EDTA Buffer (pH9.0); Permeabilization was not performed; Blocking was performed using 5% Normal Serum and 0.3% TritonX-100 for 30. minutes at room temperature. Mouse lung sections were costained with ARL13B (Proteintech #1711-1-AP) to visualize primary cilia of the lung epithelia; all sections were counterstained with DAPI Fluormount (Abcam); image attached is of mouse lung frozen tissue; due to size restrictions I am limited to this image only but am happy to provide additional images (human tissue etc.) upon request

• Applications: Immunohistochemistry, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000 (works best for IF with FFPE @ 1:500)
• Cell Tissue Type: Human lung (FFPE); mouse lung (OCT/frozen)

ACE2 Antibody Immunohistochemistry,Immunofluorescence,Other validation (1:1000 (works best for IF with FFPE @ 1:500) dilution) in Human lung (FFPE); mouse lung (OCT/frozen) (Cat no:66699-1-Ig)