CoraLite® Plus 488-conjugated ACE2 Monoclonal antibody

ACE2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IF, FC (Intra), FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F12A4

Cat no : CL488-66699

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15



Tested Applications

Positive IF detected inhuman kidney tissue
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66699 targets ACE2 in IF, FC (Intra), FC applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACE2 fusion protein Ag15554
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated Molecular Weight 805 aa, 92 kDa
GenBank Accession NumberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2883371
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACE2 antibody CL488-66699Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Adv Mater

Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy.

Authors - Feng Xie
humanIF

J Virol

The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.

Authors - Shouwen Du