CoraLite® Plus 488-conjugated ACE2 Monoclonal antibody
ACE2 Monoclonal Antibody for FC(Intra), IF
Host / Isotype
Mouse / IgG1
Human and More (1)
IF, FC(Intra), FC
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-66699
|Positive IF detected in||human kidney tissue|
|Positive FC detected in||NIH/3T3 cells|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL488-66699 targets ACE2 in IF, FC(Intra), FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||ACE2 fusion protein Ag15554|
|Full Name||angiotensin I converting enzyme (peptidyl-dipeptidase A) 2|
|Calculated molecular weight||805 aa, 92 kDa|
|GenBank accession number||BC048094|
|Gene ID (NCBI)||59272|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.
|Product Specific Protocols|
|IF protocol for CL Plus 488 ACE2 antibody CL488-66699||Download protocol|
|Click here to view our Standard Protocols|
The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.
Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy.