ACOT7 Polyclonal antibody

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  WB analysis using 15972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

ACOT7 Polyclonal Antibody for WB, ELISA

Cat No. 15972-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, human brain tissue, Jurkat cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

15972-1-AP targets ACOT7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8759

  Product name: Recombinant human ACOT7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 14-370 aa of BC017365

  Sequence: PDTCALLQPPAASAAAAPSMSGPDVETPSAIQICRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQVNVMSENILTGAKKLTNKATLWYVPLSLKNVDKVLEVPPVVYSRQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDSSQKRYRAASAFFTYVSLSQEGRSLPVPQLVPETEDEKKRFEEGKGRYLQMKAKRQGHAEPQP

• Full Name: acyl-CoA thioesterase 7
• Calculated Molecular Weight: 370 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC017365
• Gene Symbol: ACOT7
• Gene ID (NCBI): 11332
• RRID: AB_2221652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00154
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.; Authors - Shengkai Pan
human	WB,IHC	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB	J Proteomics; TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition; Authors - Wenlei Wang
human	WB,IHC	Acta Histochem; ACOT7 positively regulated by CREB1 promotes the progression of cutaneous melanoma; Authors - Ni Tang
bovine	WB,IHC,IF	Int J Mol Sci; Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis; Authors - Bin Zhou
mouse	WB	In Vitro Cell Dev Biol Anim; ACOT7 protects epidermal stem cells against lipid peroxidation; Authors - Guang Zhang