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  • KD/KO Validated

ACP5 Polyclonal antibody

ACP5 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (2)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11594-1-AP

Synonyms

ACP5, Tartrate resistant acid ATPase, TR AP, TRACP, TRAP, TrATPase, Type 5 acid phosphatase



Tested Applications

Positive WB detected inpig tonsil tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11594-1-AP targets ACP5 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACP5 fusion protein Ag2065
Full Name acid phosphatase 5, tartrate resistant
Calculated Molecular Weight 325 aa, 37 kDa
GenBank Accession NumberBC025414
Gene Symbol ACP5
Gene ID (NCBI) 54
RRIDAB_2877781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Protocols

Product Specific Protocols
WB protocol for ACP5 antibody 11594-1-APDownload protocol
FC protocol for ACP5 antibody 11594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Commun

Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.

Authors - Yinan Hu
  • KO Validated
mouse

Redox Biol

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Authors - Xiaoxiao Ji
mouseIHC

Hypertension

Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.

Authors - Jianglei Chen
mouse

ACS Appl Mater Interfaces

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Authors - Wenzheng Liao
mouseIHC

Bioact Mater

Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.

Authors - Zhuo Li
mouse

FASEB J

The function of the calcium channel Orai1 in osteoclast development.

Authors - Lisa J Robinson