ACP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACP5 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 11594-1-AP

Synonyms

ACP5, Tartrate resistant acid ATPase, TR AP, TRACP, TRAP, TrATPase, Type 5 acid phosphatase

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Validation Data Gallery

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  IHC staining of human breast cancer using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11594-1-AP (ACP5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11594-1-AP (ACP5 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of A431 using 11594-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human ACP5 (11594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

11594-1-AP targets ACP5 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACP5 fusion protein Ag2065
• Full Name: acid phosphatase 5, tartrate resistant
• Calculated molecular weight: 325 aa, 37 kDa
• GenBank accession number: BC025414
• Gene symbol: ACP5
• Gene ID (NCBI): 54
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publications

Species	Application	Title
human,mouse	IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu ; KO Validated
mouse	WB	Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
mouse	IHC	Bioact Mater; Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.; Authors - Zhuo Li
mouse	WB	FASEB J; The function of the calcium channel Orai1 in osteoclast development.; Authors - Lisa J Robinson
human	IF	Front Oncol; Single-Cell RNA Sequencing Reveals the Migration of Osteoclasts in Giant Cell Tumor of Bone; Authors - Wenyu Feng
	WB	J Cell Mol Med; Cytisine attenuates bone loss of ovariectomy mouse by preventing RANKL-induced osteoclastogenesis.; Authors - Zhi Qian