ACP5 Polyclonal antibody

ACP5 Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11594-1-AP

Synonyms

ACP5, Tartrate resistant acid ATPase, TR AP, TRACP, TRAP, TrATPase, Type 5 acid phosphatase

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Validation Data Gallery

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  FC experiment of A431 using 11594-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human ACP5 (11594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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  IHC staining of human breast cancer using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11594-1-AP (ACP5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11594-1-AP (ACP5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11594-1-AP targets ACP5 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACP5 fusion protein Ag2065
• Full Name: acid phosphatase 5, tartrate resistant
• Calculated molecular weight: 325 aa, 37 kDa
• GenBank accession number: BC025414
• Gene symbol: ACP5
• Gene ID (NCBI): 54
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publications

Species	Application	Title
mouse	IHC	Hypertension; Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.; Authors - Jianglei Chen
mouse	WB	Med Sci Monit; Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway.; Authors - Chongwen Ma
human	WB	Exp Ther Med; 4-Hexylresorcinol inhibits osteoclastogenesis by suppressing the NF-κB signaling pathway and reverses bone loss in ovariectomized mice.; Authors - Wenkai Yi
	WB	J Bone Miner Metab; miR-142-5p promotes the osteoclast differentiation of bone marrow-derived macrophages via PTEN/PI3K/AKT/FoxO1 pathway.; Authors - Zhenkai Lou
human	IHC	Dig Dis; Prognostic Significance of ACP5 in Human Gastric Cancer.; Authors - Tailai An
mouse	IHC	J Cell Biochem; (2R,3R)Dihydromyricetin inhibits osteoclastogenesis and bone loss through scavenging LPS-induced oxidative stress and NF-κB and MAPKs pathways activating.; Authors - Xuejun Zhang