ACP5 Polyclonal antibody

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  WB analysis using 11594-1-AP

  pig tonsil tissue was subjected to SDS PAGE followed by western blot with 11594-1-AP (ACP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11594-1-AP (ACP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ACP5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11594-1-AP

Publications(30)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ACP 5, EC:3.1.3.2, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP

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Tested Applications

• Positive WB detected in: pig tonsil tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

11594-1-AP targets ACP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACP5 fusion protein Ag2065
• Full Name: acid phosphatase 5, tartrate resistant
• Calculated Molecular Weight: 325 aa, 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC025414
• Gene Symbol: ACP5
• Gene ID (NCBI): 54
• RRID: AB_2877781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13686
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publications

Species	Application	Title
mouse,human	IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu ; KO Validated
mouse	WB	Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
mouse	IHC	Hypertension; Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.; Authors - Jianglei Chen
mouse	WB	ACS Appl Mater Interfaces; Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis; Authors - Wenzheng Liao
mouse	IHC	Bioact Mater; Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.; Authors - Zhuo Li
mouse	WB	iScience; StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway; Authors - Shengji Zhou