Filter: AllWBIHCIF

WB analysis of PC-3 using 15840-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15840-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15840-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15840-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15840-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 15840-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).
IF Staining of PC-3 using 15840-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15840-1-AP (ACPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ACPP Polyclonal antibody

Name: ACPP Polyclonal antibody
Brand: Proteintech
SKU: 15840-1-AP

ACPP Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 15840-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected in	human prostate cancer tissue, human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

15840-1-AP targets ACPP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8592 Product name: Recombinant human ACPP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-373 aa of BC007460 Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM Predict reactive species
Full Name	acid phosphatase, prostate
Calculated Molecular Weight	418 aa, 48 kDa
Observed Molecular Weight	50-60 kDa
GenBank Accession Number	BC007460
Gene Symbol	ACPP
Gene ID (NCBI)	55
RRID	AB_2222099
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15309
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Protocols

Product Specific Protocols
IF protocol for ACPP antibody 15840-1-AP	Download protocol
IHC protocol for ACPP antibody 15840-1-AP	Download protocol
WB protocol for ACPP antibody 15840-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
chicken	IHC	Biol Reprod Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas. Authors - Hyocheol Bae View Article
mouse	IHC	Am J Pathol PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer. Authors - Wen-Cheng Chung View Article
mouse	WB,IF,IP	Eur J Pain Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice. Authors - Xiaoxin Zhou View Article
Rat	IHC, IF	Biochem Biophys Res Commun Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats. Authors - Whasun Lim View Article