ACSBG1 Polyclonal antibody

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  WB analysis of mouse testis using 16077-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16077-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16077-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16077-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACSBG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16077-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16077-1-AP targets ACSBG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSBG1 fusion protein Ag9043
• Full Name: acyl-CoA synthetase bubblegum family member 1
• Calculated Molecular Weight: 724 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC009289
• Gene Symbol: ACSBG1
• Gene ID (NCBI): 23205
• RRID: AB_2878214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96GR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Endocrinology; mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms; Authors - Yusuf Ali