ACSL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACSL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 20710-1-AP

Synonyms

EC:6.2.1.3, EC:6.2.1.2, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 3

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Validation Data Gallery

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  WB analysis of HEK-293 using 20710-1-AP

  WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells.

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  WB analysis using 20710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 20710-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 20710-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, HuH-7 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 4 publications below

Product Information

20710-1-AP targets ACSL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: acyl-CoA synthetase long-chain family member 3
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_004457
• Gene Symbol: ACSL3
• Gene ID (NCBI): 2181
• RRID: AB_10697670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

Publications

Species	Application	Title
human,mouse	WB,IHC	Cancer Commun (Lond); Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma; Authors - Xiaoteng Cui
human	WB	Clin Transl Med; NAT10: An RNA cytidine transferase regulates fatty acid metabolism in cancer cells; Authors - Mahmood Hassan Dalhat
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
		Sci Rep; Morphologically and Functionally Distinct Lipid Droplet Subpopulations.; Authors - Shuyan Zhang
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics.; Authors - Yaqin Deng
human	WB	J Cell Sci; Exploring the ATG9A interactome uncovers interaction with VPS13A; Authors - Alexander R van Vliet