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ACSL3 Polyclonal antibody

ACSL3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20710-1-AP

Synonyms

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

20710-1-AP targets ACSL3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acyl-CoA synthetase long-chain family member 3
Calculated molecular weight 80 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberNM_004457
Gene symbol ACSL3
Gene ID (NCBI) 2181
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

Protocols

Product Specific Protocols
WB protocol for ACSL3 antibody 20710-1-APDownload protocol
IF protocol for ACSL3 antibody 20710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
humanWB

J Appl Toxicol

Changes of lipid profiles in human umbilical vein endothelial cells exposed to zirconia nanoparticles with or without the presence of free fatty acids.

Authors - Xiangjun Cheng
humanWB

Chem Biol Interact

Transcriptomic analysis suggested the involvement of impaired lipid droplet biogenesis in graphene oxide-induced cytotoxicity in human umbilical vein endothelial cells.

Authors - Yingmei Luo
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics.

Authors - Yaqin Deng

Sci Rep

Morphologically and Functionally Distinct Lipid Droplet Subpopulations.

Authors - Shuyan Zhang