ACSL4/FACL4 Monoclonal antibody, PBS Only

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  WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66617-1-Ig (same clone as 66617-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66617-1-Ig (same clone as 66617-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66617-1-Ig (same clone as 66617-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66617-1-Ig (same clone as 66617-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 66617-1-PBS

Clone No.1H5D3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

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Product Information

66617-1-PBS targets ACSL4/FACL4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18085

  Product name: Recombinant human ACSL4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 143-236 aa of BC034959

  Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY

• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated Molecular Weight: 711 aa, 79 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC034959
• Gene Symbol: ACSL4
• Gene ID (NCBI): 2182
• RRID: AB_2881977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60488
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Acyl-CoA synthetase long-chain family 4 (ACSL4) is a key enzyme that catalyzes long-chain fatty acids activation, and abnormal expression of ACSL4 is closely related to various biological responses, including steroidogenesis, inflammation response, cell death, immune activation response, and so on. Specifically, ACSL4 participates in ferroptosis, a promising target for tumor therapeutics.