ACSL4/FACL4 Monoclonal antibody

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  WB analysis using 66617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66617-1-Ig

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells.

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  WB analysis of HeLa using 66617-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66617-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 66617-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66617-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66617-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens).

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  FC experiment of HEK-293T using 66617-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 66617-1-Ig

Clone No.1H5D3

Publications(35)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Caco-2 cells, COLO 320 cells, L02 cells, HT-29 cells
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

66617-1-Ig targets ACSL4/FACL4 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, bovine, sheep
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18085

  Product name: Recombinant human ACSL4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 143-236 aa of BC034959

  Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY

• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated Molecular Weight: 711 aa, 79 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC034959
• Gene Symbol: ACSL4
• Gene ID (NCBI): 2182
• RRID: AB_2881977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60488
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family 4 (ACSL4) is a key enzyme that catalyzes long-chain fatty acids activation, and abnormal expression of ACSL4 is closely related to various biological responses, including steroidogenesis, inflammation response, cell death, immune activation response, and so on. Specifically, ACSL4 participates in ferroptosis, a promising target for tumor therapeutics.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Death Differ; Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4; Authors - Wei Liu
human	WB	Cell Death Dis; RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis; Authors - Xing Zhou
human	WB	J Cell Biol; The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance; Authors - Shan Huang
human	WB	J Med Chem; Seco-Lupane Triterpene Derivatives Induce Ferroptosis through GPX4/ACSL4 Axis and Target Cyclin D1 to Block the Cell Cycle.; Authors - Haohao Wang
human	WB,IHC	Int Immunopharmacol; Ischemia-inhibited ferric chelate reductase 1 improves ferroptosis-mediated intestinal ischemia injury via Hippo signaling; Authors - Pengzhen Liang
human	WB	Bioorg Chem; Discovery of novel benzo[b][1,4]oxazine derivatives as ferroptosis inhibitors; Authors - Bi-Wen Wan