ACSL4 Monoclonal antibody

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• KD/KO Validated

ACSL4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H5D3

Cat no : 66617-1-Ig

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Validation Data Gallery

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  WB analysis of HepG2 using 66617-1-Ig

  WB result of ACSL4 antibody (66617-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells.

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  WB analysis using 66617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Caco-2 cells, COLO 320 cells, HepG2 cells, L02 cells, HT-29 cells
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

66617-1-Ig targets ACSL4 in WB, IHC,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACSL4 fusion protein Ag18085
• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated molecular weight: 711 aa, 79 kDa
• Observed molecular weight: 74 kDa
• GenBank accession number: BC034959
• Gene symbol: ACSL4
• Gene ID (NCBI): 2182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	FEBS Open Bio; Lipoxin A4 protects primary spinal cord neurons from Erastin-induced ferroptosis by activating the Akt/Nrf2/HO-1 signaling pathway.; Authors - Na Wei