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ACSL4 Antibody

ACSL4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5D3

Cat no : 66617-1-Ig

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inHeLa cells, Caco-2 cells, COLO 320 cells, HepG2 cells, L02 cells, HT-29 cells
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66617-1-Ig targets ACSL4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACSL4 fusion protein Ag18085
Full Name acyl-CoA synthetase long-chain family member 4
Calculated molecular weight 711aa,79 kDa
Observed molecular weight 74 kDa
GenBank accession number BC034959
Gene symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSL4 antibody 66617-1-IgDownload protocol
IHC protocol for ACSL4 antibody 66617-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols