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ACSL4 Monoclonal antibody
ACSL4 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1H5D3
Cat no : 66617-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Caco-2 cells, COLO 320 cells, HepG2 cells, L02 cells, HT-29 cells |
Positive IHC detected in | human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:3000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Sample-dependent, check data in validation data gallery |
Product Information
66617-1-Ig targets ACSL4 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACSL4 fusion protein Ag18085 |
Full Name | acyl-CoA synthetase long-chain family member 4 |
Calculated molecular weight | 711 aa, 79 kDa |
Observed molecular weight | 74 kDa |
GenBank accession number | BC034959 |
Gene symbol | ACSL4 |
Gene ID (NCBI) | 2182 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ACSL4 antibody 66617-1-Ig | Download protocol |
IHC protocol for ACSL4 antibody 66617-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |