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ACSL5 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 15708-1-AP

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Con: 23 μg/150 μl

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Tested Applications

Applications:
WB, IP, ELISA
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ACSL5 Antibody WB mouse brain tissue 15708-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 15708-1-AP(ACSL5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours

ACSL5 Antibody IP mouse brain tissue 15708-1-AP

IP Result of anti-ACSL5 (IP:15708-1-AP, 4ug; Detection:15708-1-AP 1:600) with mouse brain tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, HeLa cells, mouse liver tissue
Positive IP detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACSL5 fusion protein Ag8258
Full name:
acyl-CoA synthetase long-chain family member 5
Calculated molecular weight:
76 kDa
Observed molecular weight:
66-70 kDa
GenBank accession number:
BC007985
Gene ID (NCBI):
51703
Gene symbol
ACSL5
Synonyms
ACS2, ACS5, ACSL5, FACL5, LACS 5

Background

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.


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